Determination of fungi and their aflatoxins in embryonated eggs a production batch
Keywords:
Mycotoxins, Aflatoxin, Embryonated eggsAbstract
The fungal load of origin and the presence of aflatoxins were determined in a batch of embryonated egg production, for this, aliquots of dissolution medium were seeded after a gentle rubbing of the eggshells of a batch, were incubated and then the total load of fungi of origin was quantified. Subsequently, the presence of any of the four main aflatoxins (B1, B2, G1 and G2) was determined using the ELISA method following the kit recommendations. All of the embryonated eggs had the presence of fungus, the fungal loads varied between 100 and 10 520 MFU/egg. 76 % (38/50) of the samples were positive for any of the four main aflatoxins and the estimated concentration of aflatoxins was 5.4 ± 2.9 µg/egg. It was possible to demonstrate the fail to the quality and safety of the food, both for human or animal consumption, or for animal production, since there is evidence of the high embryonic mortality generated by the mycotoxins that manage to enter the egg, in addition, it is evident the need to develop "organic" strategies for fungal control in the embryonic eggshell.
References
Alaniz-De La O, R., Juan-Morales, A. L. & Rosas-Barbosa, B. T. 2016. Egg. In: Torres-Vitela, M. R. 2016. Food safety (Seguridad alimentaria). Ed. Universidad de Guadalajara. p. 187-204.
Alizadeh, A. M., Hashempour-Baltork, F., Khaneghah, A. M., & Hosseini, H. 2020. New perspective approaches in controlling fungi and mycotoxins in food using emerging and green technologies. Current Opinion in Food Science. https://doi.org/10.1016/j.cofs.2020.12.006
Alonso, V. A., Pereyra, C. M., Keller, L. A. M., Dalcero, A. M., Rosa, C. A. R., Chiacchiera, S. M., & Cavaglieri, L. R. 2013. Fungi and mycotoxins in silage: An overview. Journal of Applied Microbiology, 115(3), 637-643. https://doi.org/10.1111/jam.12178
Bunker, M. E., Elliott, G., Heyer-Gray, H., Martin, M. O., Arnold, A. E., & Weiss, S. L. 2021. Vertically transmitted microbiome protects eggs from fungal infection and egg failure. Animal microbiome, 3(1), 1-13. https://link.springer.com/article/10.1186/s42523-021-00104-5
Chousalkar, K. K., Khan, S., & McWhorter, A. R. 2020. Microbial quality, safety and storage of eggs. Current Opinion in Food Science, 38, 91-95. https://doi.org/10.1016/j.cofs.2020.10.022
Egbuta, M. A., Mwanza, M., & Babalola, O. O. 2017. Health risks associated with exposure to filamentous fungi. International Journal of Environmental Research and Public Health, 14(7),719. https://doi.org/10.3390/ijerph14070719
Escrivá, L., Font, G., Manyes, L., Berrada, H. 2017. Studies on the presence of mycotoxins in biological samples: An overview. Toxins 9, 251. https://doi.org/10.3390/toxins9080251
Peivasteh-Roudsari, L., Pirhadi, M., Shahbazi, R., Eghbaljoo-Gharehgheshlaghi, H., Sepahi, M., Mirza-Alizadeh, A. & Jazaeri, S. 2021. Mycotoxins: Impact on health and strategies for prevention and detoxification in the food chain. Food Reviews International, 1-32. https://doi.org/10.1080/87559129.2020.1858858
Flórez-Valencia, J. S. 2020. Comparison of productive parameters of laying hens from two poultry farms of the same company, with the genetic house management guide (Doctoral dissertation, Lasallian University Corporation).
Gros, R. V., Nichita, I., Șereș, M., Ilie, M. S., Marcu, A., Cucerzan, A., Tîrziu, E. 2015. Study of the fungi dynamics in a poultry house with permanent litter. Lucr. St. Med. Vet. 48, 2572–2662.
Manders, T. T. M., Matthijs, M. G. R., Veraa, S., van Eck, J. H. H., & Landman, W. J. M. 2021. Success rates of inoculation of the various compartments of embryonated chicken eggs at different incubation days. Avian Pathology, 50(1), 61-77. https://doi.org/10.1080/03079457.2020.1834503
Magan, N., & Olsen, M. (Eds.). 2004. Mycotoxins in food: Detection and control. Woodhead Publishing.
Neira-Solís, C. 2016. Microbiota in eggs and derivatives: identification and development (Master's Degree Dissertation in Food Biotechnology. Oviedo University).
Nyholm, S. V. 2020. In the beginning: Egg–microbe interactions and consequences for animal hosts. Philosophical Transactions of the Royal Society B, 375(1808), 20190593. https://doi.org/10.1098/rstb.2019.0593
Ogunbanwo, S. T., Sanni, A. I., & Onilude, A. A. 2003. Influence of cultural conditions on the production of bacteriocin by Lactobacillus brevis OG1. African Journal of Biotechnology, 2(7),179-184. https://www.ajol.info/index.php/ajb/article/view/14779
Prapagdee, B., Kuekulvong, C., & Mongkolsuk, S. 2008. Antifungal potential of extracellular metabolites produced by Streptomyces hygroscopicus against phytopathogenic fungi. International Journal of Biological Sciences, 4(5),330. https://doi.org/10.7150/ijbs.4.330
Piskorska-Pliszczynska, J.; Mikolajczyk, M.; Warenik-Bany, M.; Maszewski, S.; Strucinski, P. 2014. Soil as a source of dioxin contamination in eggs from free-range hens on a Polish farm. Sci. Total Environ. 145, 435–436. https://doi.org/10.1016/j.scitotenv.2013.07.061
Ráduly, Z., Szabó, L., Madar, A., Pócsi, I., & Csernoch, L. 2020. Toxicological and medical aspects of Aspergillus-derived mycotoxins entering the feed and food chain. Frontiers in microbiology, 10, 2908. https://doi.org/10.3389/fmicb.2019.02908
Rodríguez Orozco, A. R., Vargas Villegas, E., Tafolla Muñoz, L., Ruiz Reyes, H., Hernández Chávez, L. A., & Vázquez Garcidueñas, S. 2008. Fungal genera isolated from patients with allergic rhinitis and their relationship with the subcutaneous prick hypersensitivity test. Revista mexicana de micología, 28(SPE), 89-94. http://www.scielo.org.mx/scielo.php?pid=S0187-31802008000300011&script=sci_abstract&tlng=en
SCIENCE. 2021. Course Hero. Relative humidity values using saturated salts. University of Texas. Retrieved the; 08/18/2021. Available at: https://www.coursehero.com/file/p4r2pu8k/Tabla-2-Valores-de-humedad-relativa-usando-sales-saturadas-valores-tomados-de/
Shah, P. A., & Pell, J. K. 2003. Entomopathogenic fungi as biological control agents. Applied Microbiology and Biotechnology, 61(5), 413-423. https://link.springer.com/article/10.1007/s00253-003-1240-8?LI=true>
Smaoui, S., Elleuch, L., Bejar, W., Karray-Rebai, I., Ayadi, I., Jaouadi, B. & Mellouli, L. 2010. Inhibition of fungi and gram-negative bacteria by bacteriocin BacTN635 produced by Lactobacillus plantarum sp. TN635. Applied Biochemistry and Biotechnology, 162(4), 1132-1146. https://link.springer.com/article/10.1007/s12010-009-8821-7
Szablewski, T., Stuper, K., Cegielska-Radziejewska, R., Kijowski, J., Perkowski, J. 2010. Ergosterol as an indicator of the presence of microscopic fungi in eggs for human consumption produced in different husbandry systems. Poult. Sci. 89, 2491–2493. https://doi.org/10.3382/ps.2009-00366
Takaya, N. 2002. Dissimilatory nitrate reduction metabolisms and their control in fungi. Journal of Bioscience and Bioengineering, 94(6), 506-510. https://doi.org/10.1016/S1389-1723(02)80187-6
Thines, E., Heidrun, A. N. K. E., & Weber, R. W. 2004. Fungal secondary metabolites as inhibitors of infection-related morphogenesis in phytopathogenic fungi. Mycological research,108(1),14-25. https://doi.org/10.1017/S0953756203008943
Tomczyk Ł, Stępień Ł, Urbaniak M, Szablewski T, Cegielska-Radziejewska R, Stuper-Szablewska K. 2018. Characterization of the mycobiota on the shell surface of table eggs acquired from different egg-laying hen breeding systems. Toxins 10(7):293. https://doi.org/10.3390/toxins10070293
Weaver, A. C., Adams, N., & Yiannikouris, A. 2020. Invited Review: Use of technology to assess and monitor multimycotoxin and emerging mycotoxin challenges in feedstuffs. Applied Animal Science, 36(1), 19-25. https://doi.org/10.15232/aas.2019-01898
