Effect of the Biosynthesis of Silver Nanoparticles (AgNPs) and Lithium (LiNPs), obtained by reduction with Quercetin, on the proliferation of a strain of Staphylococcus aureus (ATCC 25923) and Klebsiella pneumoniae (ATCC 700603)
Keywords:
nanomaterials, morphology, antimicrobial activity, green synthesisAbstract
Introduction: The growing resistance to antibiotics has led to the design and evaluation of new alternatives against various pathogens of clinical interest, including Staphylococcus aureus Klebsiella pneumoniae. Among these alternatives are the so-called metallic nanoparticles, which have exhibited interesting antimicrobial potential. Objective: The study aimed to evaluate the effect of the biosynthesis of silver nanoparticles (AgNPs) and Lithium (LiNPs) on the proliferation of a strain of S. aureus and K. pneumoniae. Methodology: AgNPs and LiNPs were synthesized using lithium and silver salts and quercetin as a reducing agent. These NPs were characterized by scanning electron microscopy (SEM). To evaluate the antimicrobial effect of the NPs, a range of concentrations was established (0.2; 0.4; 0.6; 0.8; and 1 mg/mL). The bacteria were exposed to these concentrations by taking optical density readings every 60 min for 6 h at 600 nm and one at 24 h to calculate the minimum inhibitory concentration (MIC).Results: The AgNPs had a size of 38 nm and 36 nm for the LiNPs, both with spherical morphology and certain aggregations. For all the concentrations evaluated, inhibition of bacterial growth was observed with a minimum inhibitory contraction (MIC) close to 0.8 mg/mL-AgNPs and 0.6 mg/mL-LiNPs against S. aureus and 1 mg/mL- AgNPs and 0.6 mg/mL-LiNPs against K. pneumoniae. Conclusions: The biosynthesis of metallic nanoparticles constitutes a future alternative for the treatment of bacterial infections. AgNPs and LiNPs exhibited excellent performance against S. aureus and K. pneumoniae over the entire range of concentrations.
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