Use of native yeasts as biocontrol agents for phytopathogenic fungi in grapes (Vitis vinifera subsp. vinifera L.)

Authors

  • Mariano Giralda-Arellano Instituto Tecnológico de La Paz, México
  • Domingo Barrios-Acosta Instituto Tecnológico de La Paz, México

Keywords:

Vitis vinifera, Fungal diseases, Biocontrol, Native yeasts

Abstract

The vine plant is susceptible to attack by fungal diseases caused by various species of filamentous fungi, yeasts and some bacteria. In Baja California Sur, Mexico, the incidence and severity of fungal infections in grapevines increased in the last two vegetative cycles. Biological control through the use of naturally found microorganisms that antagonize postharvest pathogens has been developed as an alternative to the use of chemical fungicides. Objective of this study was to study the antagonistic effects of native yeast isolates on filamentous fungi of agronomic and economic interest. Filamentous fungi and yeasts were isolated from grape grains. The antagonistic activity of isolated yeasts against isolated filamentous fungi was determined. The test was performed first in vitro at 25 ° C and then in vivo at 25 ° C and 2 ° C (only Botrytis cinerea, due to its ability to grow at low temperatures). In total, 16 yeasts and 6 filamentary fungi were isolated: Botrytis cinerea, Aspergillus caelatus, Aspergillus carbonarius, Penicillium comune, Rhizopus setolonifer and Plasmopara viticola. In vitro and in vivo antagonism tests at 25 ° C showed that fermenting musts were the main viticultural environment from which antagonist yeasts (mostly S. cerevisiae species) capable of suppressing isolated phytopathogenic fungi were isolated. In the case of B. cinerea, the in vivo test was also carried out at 2 ° C, but no antagonist isolation reduced the percentage of incidence of gray rot by more than 50%. B. cinerea, A, carbonarius and R. stolonifer showed sensitivity to the action of 8 antagonist yeast isolates (5 belonging to the genus Saccharomyces and 3 to non-Saccharomyces). The isolates of the species S. cerevisiae (isolated mainly from fermentation musts) showed a high antagonistic capacity to suppress the different filamentous fungi under in vitro and in vivo conditions. Some biosuppressive yeasts were able to present a broad antagonistic spectrum against filamentous fungi.

References

Carrau Magariños, M. J. 2005. Evaluación de la capacidad antagónica de microorganismos aislados de la uva frente a hongos patógenos: Botrytis spp., Plasmopara viticola, Penicillium spp. y Monilinia spp. (No. 579.67 CAR). http://www.sidalc.net/cgi-bin/wxis.exe/?IsisScript=FCT.xis&method=post&formato=2&cantidad=1&expresion=mfn=001190

Chalutz, E., & Wilson, C. L. 1990. Postharvest biocontrol of green and blue mold and sour rot of citrus fruit by Debaryomyces hansenii. Plant disease, 74(2), 134-137 https://ci.nii.ac.jp/naid/80005145452/

Del Moral, J. 2006. La sanidad de los vegetales cultivados. Caja Rural de Extremadura. España. https://www.researchgate.net/profile/Margarita-Lopez-Corrales/publication/305995917_Plagas_y_enfermedades_del_cultivo_de_la_higuera/links/57a97ce708aed1b226245814/Plagas-y-enfermedades-del-cultivo-de-la-higuera.pdf

De La Torre. B. 1990. Enfermedades de las plantas cultivadas. 5ta. ed. Alfa Omega. Chile.

Editotrial El Vigía. (2019). Domina las uvas cultivos perenes en BCS. Recuperado el: 11/02/2019. Disponible en: https://www.elvigia.net/general/2020/5/15/dominan-las-uvas-cultivos-perennes-348198.html

Giralt, L., Carrasco, C. D., Barrios, G., Catalina, O., & Aybar, J. R. (2005). Guía básica de buenas prácticas vitícolas para minimizar la presencia de ocratoxina A en los productos vitivinícolas. ACE: Revista de enología, (59), 15. https://dialnet.unirioja.es/servlet/articulo?codigo=1237926

Krugg, J. W., & Valdivieso, H. R. 2012. Aislamiento de hongos fitopatógenos en lesiones de hoja y tallo de cultivos de vid, Vitis vitícola, de Cascas, La Libertad (Perú). https://www.academia.edu/download/33102790/Aislamiento_de_hongos_fitopatogenos_en_lesiones.pdf

Ortiz Cubells, B. (2020). Evaluación del potencial antifúngico de Aloe vera frente a hongos fitopatógenos y de postcosecha (Doctoral dissertation, Universitat Politècnica de València). https://riunet.upv.es/handle/10251/157556

Ortiz Cubells, B. (2020). Evaluación del potencial antifúngico de Aloe vera frente a hongos fitopatógenos y de postcosecha (Doctoral dissertation, Universitat Politècnica de València). https://riunet.upv.es/handle/10251/157556

Rebosto X., Carrau Magariños, Paz A., Boido E., Dellacassa E., y Carrau F. M. 2006. Grapes and Vineyard Soils as Sources of Microorganisms for Biological Control of Botrytis cinerea. American Journal of Enology and Viticulture. 57(3), 332-338. https://www.ajevonline.org/content/57/3/332.short

Representación Estatal de la Secretaria de Agricultura y Desarrollo Rural (RE-SADER). 2019. Alcanzan cultivos perennes una superficie de 11,500 hectáreas en la zona costa de Baja California. Recuperado el: 19/05/2020. Disponible en: https://www.gob.mx/agricultura%7Cbajacalifornia/articulos/alcanzan-cultivos-perennes-una-superficie-de-11-500-hectareas-en-la-zona-costa-de-baja-california

Vidhayasekaran, P. 2004. Concise Encyclopedia of Plant Pathology. The Haworth Press Inc. USA. https://api.taylorfrancis.com/content/books/mono/download?identifierName=doi&identifierValue=10.1201/9781482277951&type=googlepdf

Waller, J. M., Lenné, J. M., & Waller, S. J. (Eds.). 2002. Plant pathologist's pocketbook. Cabi. https://books.google.es/books?hl=es&lr=&id=d_aqiD73NUAC&oi=fnd&pg=PR8&ots=NdITGRtD2N&sig=Z8DfmpU2--cAN6AyUYi3EtFdN_4

Published

2021-12-15

How to Cite

Giralda-Arellano, M., & Barrios-Acosta, D. (2021). Use of native yeasts as biocontrol agents for phytopathogenic fungi in grapes (Vitis vinifera subsp. vinifera L.) . Multidisciplinary &Amp; Health Education Journal, 3(1), 85–91. Retrieved from http://journalmhe.org/ojs3/index.php/jmhe/article/view/17

Issue

Section

ORIGINAL ARTICLE